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KMID : 0882419930440030285
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Korean Journal of Medicine
1993 Volume.44 No. 3 p.285 ~ p.293
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The Effects of Interleukin 4 and Interferon-gamma on the Low Affinity Receptor for IgE on Peripheral Blood B Lymphocytes
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문종호
박춘식/어수택/한동철/화응덕/이희발
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Abstract
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ackground : FceRII is one of IgE binding receptor and is generally known as the low
affinity receptor for IgE. FceRII is identified on B cells, monocytes, macrophages, Langerhan;s
cells, T cells and eosinophils. IgE upregulates FceRII, and IL-4 upregulates FcerII by
increasing the rate of synthesis of both FcerII and IgE. Interferon-r blocks IL-4-induced
upregulation of FceRII. The purpose of this study is to search the kinetics of FceRII
expression on normal human peripheral blood B cells.
Methods : We measured time and dose-dependent expression of FceRII on peripheral blood
B cells with flow-cytometry after stimulation with IL-4 and INF-r in healthy volunteers.
Results :
1) The percentage of FceRII positive B lymphocytes significantly decreased 24hrs after
incubation without stimulation when compared to that before incubation (p<0.05).
2) IL-4-induced upregulation of FceRII expression by B lymphocytes reached maximum
level at 48 to 72hrs after stimulation, then decreased gradually and was dose dependent up to
1000 u/ml.
3) IL-4 did not change the fraction of B lymphocytes in cultured PBMC.
4) The stimulating effect of IL-4 (100 u/ml) on FceRII expression of B lymphocytes was
partially suppressed by the addition of INF-r (100 u/ml).
Conclusion : IL-4 increases FceRII expression on normal peripheral blood B lymphocytes
without proliferative effect and INF-r partially inhibits IL-4 induced upregulation of FceRII.
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KEYWORD
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